Comparison of Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) and Repetitive Sequence-PCR (REP-PCR) for Differentiation of Riemerella anatipestifer Isolates
نویسندگان
چکیده
منابع مشابه
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for rapid diagnosis of neonatal sepsis
BACKGROUND & OBJECTIVES The difficulties in diagnosis of neonatal sepsis are due to varied clinical presentation, low sensitivity of blood culture which is considered the gold standard and empirical antibiotic usage affecting the outcome of results. Though polymerase chain reaction (PCR) based detection of bacterial 16S rRNA gene has been reported earlier, this does not provide identification o...
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Early growth traits in sheep are economically important. Breeding for these traits has been shown to be problematic using quantitative genetic methods, particularly in native sheep herds. Molecular genetics is useful for sheep breeding. The gene for insulin-like growth factor 1 (IGF1) is one of the important candidate genes known for ovine early growth traits. The aim of this study was to inves...
متن کاملDetection of avian reoviruses causing tenosynovitis in breeder flocks in Iran by reverse transcription-polymerase chain reaction (RT-PCR) and restriction enzyme fragment length polymorphism (RFLP)
BACKGROUND:Avian reoviruses (ARVs) are members of theOrthoreovirus genus; one of the 12 genera of the Reoviridaefamily. The ARVs are the cause of some important diseases inpoultry such as reovirus-induced arthritis, tenosynovitis,chronic respiratory disease, and mal-absorption syndrome.OBJECTIVES: In this study, the presence of ARVs in the Iranianbreeder flocks was investigated through reverse ...
متن کاملشناسایی عوامل ایجاد کننده ی کاندیدوری به روش polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) در شهر اصفهان
مقدمه: candidiasis منتشره، دیابت، حاملگی، مصرف بی رویه ی آنتی بیوتیک های وسیع الطیف، عوامل مستعد کننده ی candidiasis دستگاه ادراری می باشند. شیوع عفونت با گونه های غیر candida albicans رو به افزایش است. هدف از انجام این مطالعه، بررسی میزان شیوع گونه های candida مسبب کاندیدوری در شهر اصفهان با استفاده از روش polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) بود. روش ه...
متن کاملComparative efficacy of PCR-based restriction fragment length polymorphism (RFLP) & multiplex PCR for glycoprotein B (gB) genotyping of human cytomegalovirus.
BACKGROUND & OBJECTIVE Glycoprotein B (gB), involved in cell-to-cell transmission of human cytomegalovirus (HCMV), is a critical factor in tissue tropism and viral pathogenesis. The aim of the present study was to compare the efficiency of PCR-based RFLP and multiplex nested PCR for gB gene of HCMV to determine their genotype in clinical specimens from patients with HCMV. METHODS The PCR base...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2019
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2019.810.311